Hydroxychloroquine Protonation in Blood and Cells - inconsistent information on claimed alkalinization inside targets, emphasis on Coronavirus?

Some researchers have claimed that Hydroxychloroquine (HCQ) can enter cells and make the environment more "alkaline" by soaking up protons. Many publications discuss only 2 nitrogen atoms that can...

08 August 2020 6,262 3 View

Do rats play a part in Covid-19?

The VIZIONS project in Vietnam in 2018 examined bat and rat urine and faeces. Alpha coronavirus was found in bats, beta in rats, with a comment that rats in China also carry coronavirus. So,...

10 May 2020 7,974 5 View

Organofluorine compounds in Kombucha?

Kombucha, a fermented Tea with low pH, contains substantial Fluoride in the form of Hydrogen Fluoride as well as the ion. Kombucha has been associated with many adverse effects, including acute...

12 February 2020 408 1 View

Could areas of high Fluoride ingestion be more susceptible to Coronavirus outbreaks?

The US Government Comparative Toxicogenomics database shows that Fluoride can inhibit Human immunity to viruses and pneumonia. Angiotensin I-Converting Enzyme (ACE), 2'-5'-Oligoadenylate...

03 February 2020 6,459 4 View

Do you have Fluoride analysis of Psammoma bodies isolated from Cancers or could you have them analyzed now from stored specimens?

I am updating my earlier review on the role of Fluoride doped Hydroxyapatite in Cancer and my current focus is on Psammoma Bodies which have been found, and identifed as high risk, in a very wide...

31 January 2019 3,477 1 View

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01 March 2018 8,712 2 View

Does anyone know of a means for testing worldview assumptions (apart from the IAT) and also assessing worldview (pre-theoretical) transformation?

I am interested in testing worldviews - at the assumption/presupposition/pre-theoretical (Weltanschauung) level. (This is not a test of espoused beliefs.) Harvard's IAT seems to do this in a very...

12 February 2017 8,608 3 View

Has anyone come across a study comparing the genetic profiles of C57BL/6J mice to outbred CD-1's?

Specifically in regards to inflammation and cartilage specific genes

03 February 2016 5,689 2 View

COVID-19: Are there any promising suggestions for interim medications while a vaccine is being developed?

See...

01 January 1970 2,745 100 View

HIV segments identified with India, Thailand and Kenya in Covid-19 receptor

01 January 1970 7,209 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View