The Pfizer BioNTech jab Manufacturing Process uses modified DH10B Escherichia coli Bacteria cells to grow the DNA used to make their Covid19 Spike Protein encoding mRNA.
Has anyone found out the identity of the Endotoxins in the Pfizer Jabs caused by the Bacteria used in production? Could they include the Carcinogenic Colibactin?
DH10B does not produce colibactin. And normally endotoxins are removed during purification when an E. coli is used to produce pharmaceuticals.
Dear Pedro Morais unfortunately you are wrong on this one because we know that the EMA sets a requirement for Endotoxin measurements. Pfizer BioNTech must comply with the standard and is forced to analyze for them because they use E Coli in their production of the mRNA jabs..
I have added a table that shows the Endotoxin measurement reporting for a number of batches to the top of this discussion.
DH10b is a pretty standard K12 strain, so I imagine that would be the set produced. There might be specific data on DH10b but I am not aware of it.
Dear Michael J. Benedik
I have found that strains of DH10B E. Coli do indeed produce Colibactin
Article Escherichia coli Producing Colibactin Triggers Premature and...
Article Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
Dear Geoff Pain that paper does not actually say that. In that paper they are using DH10B to host a plasmid encoding the genes to make colibactin and the negative control is DH10B with a neutral plasmid. So DH10B itself does not produce colibactin, although yes it can if you put the right plasmid into it. But the strains used for vaccine production do not.
Thanks Michael J. Benedik I just edited my previous post to include a second reference on the pks island strain of DH10B which does produce Colibactin. So to resolve this issue we will have to find out exactly which company supplied the E Coli that Pfizer used and full genomics of the strain.
Secher et al. state that "Epidemiological surveys showed that up to 34% of these commensal B2 strains carried the pks island"
Geoff Pain your second reference says essentially the same thing as the first references (as do a few others that study colibactin). Those papers all say that DH10b does not express that activity unless it carries a large genomic island from pathogenic strains that are cloned onto a plasmid/bac which encodes those genes. Note this is not saying that some strains of DH10b have that genomic island, it says that the island is purposely introduced into DH10b on a plasmid in order to study it. So with the exception of those purpose built DH10b strains, DH10b does not express this activity.
Commensal B2 strains are quite different than K12 strains from which DH10b is derived. The quote you provide saying 34% carry pls is about B2 strains, and is not about DH10b.
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