I want to isolate endosomes and lysosomes but not through ultracentrifugation.
If someone knows a reliable kit for this purpose please let me know.
lysosome isolation kit
Http://www.sigmaaldrich.com/life-science/proteomics/protein-sample-preparation/organelle-isolation/lysosome-isolation-kit.html
yes there kits for lysozyme, you may check the website of lifetechnology and Sigma.
Years ago we have isolated organelles by FACS sorting, based on staining with fluorescently conjugated antibodies.
http://www.desatoya.com/PostersAndPresentations/CellOrganelleProteomicsEB2002.pdf
Magnetic selection with MACS(TM) beads should also work.
Best
Diether
http://www.desatoya.com
Thanks to all .I will check for these links.
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