Has anybody used the SYGNIS TruePrime™ Single Cell WGA kit?

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How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

How to fix install package meta in R?

> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’

18 July 2024 3,536 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to download fasta files in "gene features" format from NCBI with multiple samples in R?

Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...

04 July 2024 9,875 1 View

How to remove aluminium oxide from powder ?

I synthesise intermetallic compounds through nickel and aluminium powders. But the pure powder after synthesis is difficult to obtain. Especially the presence of aluminium oxide very seriously...

01 July 2024 4,030 3 View

The difference between the E. Laviron's model and RICHARD S. Nicholson's model for electrode reaction kinetics?

For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...

30 June 2024 580 3 View

Impact of nuclear-mitochondrial DNA segments (NUMTs) in phylogeny construction?

"Nuclear-mitochondrial DNA segments (NUMTs) are mitochondrial DNA (mtDNA) fragments that have been inserted into the nuclear genome." (Xue et al., 2023) I would like to know under this...

20 June 2024 603 1 View

Why do I have an increasing concentration for increasing dilutions on spectroscopy even though they should all be the same final concentration?

Hello. We have a sample with an unknown concentration of polyphenols. We're diluting the sample in order to perform the Folin Ciocalteau method. 0.2 grams sample / 4 mL 70% methanol and 30%...

10 June 2024 2,134 3 View

Why does the UV absorption spectrum of red quantum dot film not exhibit a significant absorption peak?

Why does the UV absorption spectrum of red quantum dot film not exhibit a significant absorption peak? As shown in the following Figure.

27 May 2024 4,288 3 View

Housekeeping protein for serum?

As the title states, I am looking for a housekeeping protein for serum (for Western blots). This research is in the context of pregnancy so it has to take into account normal vs diseased (e.g....

15 May 2024 9,219 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View