Yunfu Lin

4 Questions 15 Answers 0 Followers

Questions related from Yunfu Lin

How efficient is Taq polymerase in low temperature?

I wonder the efficiency of Taq polymerase at low temperature such as around 60 degrees. Has anybody tried?

17 April 2015 236 7 View

What is the best way to completely remove primers from PCR products?

I have tried to use PCR cleaning kit from Qiagen, and it can efficiently but not completely remove the primers. Do you have other suggestions, such as Exo I digestion and so on? Thanks a lot.

17 April 2015 4,651 10 View

Has anybody used the SYGNIS TruePrime™ Single Cell WGA kit?

If you have used, can you share your experience with me? Thanks a lot.

21 January 2015 2,846 2 View

What are the potential sources of contamination during whole genome amplification?

I wonder anyone has the experience that DNA contamination can be from the reagents, plastic supplies, experimental equipments, or even the construction materials during single cell...

06 December 2014 1,633 9 View