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Hello, I have a solution wih a DNA fragment with a sticky-end cut. It is possible ligate the oligo to this extreme without anneal it with the complementary? Thnak you
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Hello, I have a mix of 10 fragments generated with 2 endonucleases (1 produces blunt end and other , PSTI, produce overhang ends): each fragment has a blunt and and overhang end. I want ligate a...
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