Has anybody used pronase digestion on the cell culture media? if yes, could you please provide me a brief protocol?

Nazli Khodayari @Nazli_Khodayari

11 November 2017 1 9K Report

has anybody used pronase digestion on the cell culture media? if yes, could you please provide me a brief protocol?

Yassine Ouhaddi

I use pronase digestion to extract chondrocytes from cartilage.

Preparation of pronase: 500mg in 500ml DMEM (10% FBS + 1Ab), Filter with a 0.2μm, then aliquots in 25ml.

I treat my cartilage with 25 ml of pronase for 1H in a 37 ° C shaker.

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