Does any body knows if we can get stable transfection out of the PLVX-Tet-on plasmid which was designed originally for lentivirus production?

01 January 2017 1 9K Report

stable cell line production

It should be possible. Lentiviral vectors are designed to be integrated into the genome by the virus so I assume your vector has a selection marker that will allow you to select positive cells. I would suggest you linearize your plasmid before the transfection in order to improve the efficiency.

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Science method

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