RNAiMax is usually used for siRNA transfection but if anybody have used for transfecting peptides into cells please let me know.
I personally never heard of it but I am not working in that field. But, I have used a few small peptides before and realized that as long as it is small (~15 a.a) the it may have the chance to go into cells or you can engineer tat into the peptide than can be of help if the peptide is bigger.
thanks Teng
i have only 10aa but still it is finding difficult going inside and I do not want to add modifications like Myr or Palmitoylation to the peptide just to avoid repeating the activity of the peptide once again, however that will be my last option.
Do not know the detail about the peptide, charge, and conformation so it will be hard to say. I used to work with D4F/L4F which I recall is about 14 a.a.. Tat tag is about 12 a.a. and always guarantee entering into the cell.
Just talked to Professor Kirkwood A. Pritchard Jr. a few minutes ago. His recommendation is to use PEP-1 which can carry your peptide into cells easily. Otherwise tat is another good option, as I have mentioned before but using a few glycine to link them. To prove the peptide entering the cell you can lable with fluorescent probe or link the peptide with biotin the prove the entering by avidin-HRP. You can contact Professor Pritchard for help and he is super nice. Best.
thanks Venkatesh i am right now trying DeliverX let me see how it goes i will keep you all posted. Meanwhile if anybody have used DeliverX please provide your experience
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