Francesco Gobbo

3 Questions 32 Answers 0 Followers

Questions related from Francesco Gobbo

Has anybody experience in purifying synaptosomes from mouse brain by affinity tag?

I need to purify a class of synaptosomes (so I can start from a synaptosome preparation with conventional methods) by affinity tag. The tag would be 3xFLAG -TEV-HisTag and is in the extracellular...

05 October 2017 5,590 4 View

Do you know any antibodies for synapse markers?

Could you suggest any good marker and corresponding antibody you employed to mark synapses? PSD95 seems a suitable option. I am using it on primary neuron culture from mouse cortex and...

04 March 2015 2,578 12 View

Do lentiviral vectors retain introns?

I am wondering whether RNA-based vectors like lentivirus can be used to transfer intron-containing transgenes. Being RNA-based, one could fear that introns are spliced out from the virion genome....

11 January 2015 3,639 13 View