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Questions related from Francesco Gobbo
I need to purify a class of synaptosomes (so I can start from a synaptosome preparation with conventional methods) by affinity tag. The tag would be 3xFLAG -TEV-HisTag and is in the extracellular...
05 October 2017 5,586 4 View
Could you suggest any good marker and corresponding antibody you employed to mark synapses? PSD95 seems a suitable option. I am using it on primary neuron culture from mouse cortex and...
04 March 2015 2,573 12 View
I am wondering whether RNA-based vectors like lentivirus can be used to transfer intron-containing transgenes. Being RNA-based, one could fear that introns are spliced out from the virion genome....
11 January 2015 3,635 13 View