Has anybody done citrate buffer retrieval for TUNEL coupled with/without IHC?

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Manal T. Hussein

I used the Tunnel kit before from Roche it was very goo. I did cooking with citrate buffer instead of Proteinase K and it worked

Duke Bayar

thanks!

Jordan R. Yaron

We routinely used citrate buffer for TUNEL/IHC.

it's weird because I've tried CITRATE for TUNEL and got inconsistent results with this S7101 kit from millipore. has anybody tried a double staining - TUNEL & IHC?

Duke, that's what we routinely use it for. Dual staining of TUNEL with IHC.

Jordan, is it the same kit? can i ask what tissue and what you stain for in the IHC? and how you develop your IHC?