Can any body advice if there is a protocol for calculating sample size from one group of sheep?

can any body advice if there is a protocol for calculating sample size from one group of sheep, population of 100 for instance? if there any software can i apply to get the minimum and maximum...

09 October 2016 6,500 6 View

Can anybody suggest the best way of increasing the specificity of Ryan 2013 nasted primers for 18S rRNA of Cryptosporidium spp detection?

can anybody suggest the best way of increasing the specificity of Ryan 2013 nasted primers for 18S rRNA of Cryptosporidium spp detection? in other word, that primer cross reacting with other...

05 June 2016 3,937 1 View

Can anybody suggest a useful tool for designing qPCR primers?

I am wondering if I could find reliable software of designing primers for qPCR

03 April 2016 6,316 5 View

How can I prepare intestinal tissue from rodent for Cryptosporidium detection?

Is there anybody tried preparing intestinal tissue from rodent for identification of Cryptosporidium? please advise.

02 March 2016 3,680 3 View

Cryptosporidium DNA works first time after extraction but did not work for next time?

10 November 2015 6,344 5 View

Has anyone tried using FTA cards for cryptosporidium DNA extraction? Is it possible subjecting fecal samples to FTA cards in other words?

Have anyone tried using FTA cards for Cryptosporidium DNA extraction? in other words, is it possible subjecting fecal samples to FTA cards? i hope to find answer to this question since my research...

03 April 2015 7,263 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View