Hacat cell lines are not Available can we use A 431 cell lines
Dear Ravindra,
HaCaT is a normal keratinocyte cell derived from human skin but immortalized whereas A431 cells are epidermoid carcinoma cells. Although HaCaT cells also show epidermal-like organization, I doubt you can use A431 in place of it. But it all depends on what kind of experiment you have in mind. Maybe if you share then I would be able to suggest something better.
For your information, NCCS Pune has HaCaT cells and recently someone from our lab got it from there.
Good day!
Hello Ravindra Ganpati Gaikwad
You should not use A 431 cells because the behavior of both A 431 and HaCaT cell lines will be different in culture. HaCaT cells are spontaneously immortalized, human keratinocyte cell line acquiring the ability to proliferate indefinitely and can be cultured for several generations. Though they are immortalized, they will still possess the physiological properties of normal cells like requiring normal growth factors for proliferation, show contact inhibition though generally to a lesser extent than normal cells, retain normal growth responses to serum and so on and so forth.
On the other hand, A431 which is an epidermoid carcinoma cell line is a hyper triploid human cell line. A431 will behave entirely different in culture because this cell line has been derived from a clinical tumor. So, it will have several oncogenic mutations as well as abnormal karyotype which will cause it to behave differently than an immortalized cell line like HaCaT cell line.
So, I recommend you to use HaCaT cell line for your experimental work. As mentioned above this cell line could be available at NCCS.
I hope this is helpful.
Best Wishes.
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