Can you use the Normalized Difference Soundscape Index (NDSI) as a measure of anthropogenic noise?

I am working on a past project in which I recorded the ambient noise in each cardinal direction for a minute but I did not obtain the LEQ sound pressure levels at the time I was recording the...

29 November 2020 8,138 2 View

Can anyone suggest me in vivo assay for capturing long non coding RNA and its interacting partner miRNA?

On the basis of in-silico analysis we hypothesized that a long non-coding (lncRNAs) interacts with miRNA thus inhibiting that miRNA function in bread wheat. Now I want to isolate this lncRNA-miRNA...

12 August 2020 7,643 2 View

Dunn method to calculate diffusive and capacitive contributions for electrode material?

Discussions

15 May 2020 6,841 20 View

How can one do slope stability analysis in Plaxis or RS2 for debris slopes, during and after rainfall if the rainfall intensity is known??

How can one do slope stability analysis in Plaxis or RS2 for debris slopes, during and after rainfall if the rainfall intensity is known?

21 February 2020 6,074 2 View

How to define the Interface property?

Hi, I am modeling a reinforced concrete beam in DIANA FEA. I used steel plates for the supports and loading plates. So how can I define interface property between the concrete and the loading...

18 September 2019 2,052 1 View

STOP error in factorization: (In BoltzTraP) How to solve for SOC?

15 October 2018 2,757 4 View

Why there is a change in the trend of the main effect of Garnet size on the depth of cut in the abrasive waterjet machining process?

Am working on the abrasive waterjet machining of Brass, during the analysis of variances, it was found that when the abrasive grain size was decreased from 60mesh to 80mesh, there was decreasing...

24 July 2018 6,947 9 View

Active mass loading preferable for supercapacitor electrode?

Discussions

04 October 2017 4,854 25 View

Significance of HRTEM Fast Fourier transform (FTT) about crystal structure in comparison with SAED pattern?

Discussions

21 June 2017 7,742 13 View

Reasonable scan rate/current density should be used whether reporting maximum specific capacitance for supercapacitor?

Discussions

08 June 2017 5,567 6 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View