Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it OK??

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it ok??

25 January 2021 5,336 10 View

How can solve the second order linear homogeneous partial differential equation with variable coefficients?

can someone help me with reduced the partial differential equation du/dt = - f(x) d^2 u/ dx^2 - g(x) du/dx --- (1) to become: du/dt = - p(x) d^2 u/ dx^2...

21 January 2021 3,296 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

Selection of research topic for phd in management any one suggest me what should be topic of research for phd thesis ?

i want guidance from seniors that what is field of research in management, marketing field which is important in present era please suggest

01 January 2021 3,901 4 View

Any recommendation to calculate time to dominant design emergence?

Any recommendation to calculate time to dominant design emergence?

01 January 2021 3,809 5 View

Are drones following the e-Auto industry technological trajectory or are they following its own?

* Are drones following the e-Auto industry technological trajectory or it is following its own? * Will drones diffuse faster than electrical vehicles? * Will it depend on the application?, What...

29 December 2020 7,379 3 View

Parameters for Social Media Viability?

I am working on social media viability for political campaigns, so your suggestions are highly appreciated what parameters should use to analyze Social media viability for political campaigns

27 December 2020 9,010 2 View

How to explain insignificant independent variable which is significant with interacting (Moderation) variable?

10 December 2020 3,037 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View