Can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane?

can we use Comassie blue for loading control after the immunoblotting and imaging of the primary of interest on membrane? Is it reversible?

30 March 2016 875 5 View

How do i prepare the positive control from a purified peptide for western blot?

how do i prepare the positive control from a purified peptide for western blot?

13 December 2015 942 4 View

What software can be used for docking of proteins with DNA?

What software can be used for docking of proteins with DNA? I've heard that AutoDock can be used for docking proteins and RNA but does it work with DNA as well? And are there any other softwares?

10 April 2013 6,593 2 View

Reason for spurious colonies on kanamycin plates.

I've cloned a 1kb fragment into pet28a vector. Along with my positive colonies, I find a lot of white spurious colonies growing on the plates. These colonies are much smaller than my dh5a colonies...

25 March 2013 7,903 28 View

Does there exist any correlation between Protein Size and Resolution of it's X-Ray crystal Structure?

I see that the resolution of X-ray solved protein structures from the PDB (the whole of PDB) has a very high correlation with the size of the protein structures. I guess, this is nothing new but...

13 June 2012 4,496 2 View

SPSS Binary Regression

I am running a binary regression in SPSS with one dependent variable and 5 independent variables. The results show no values for 3 of these variables but I am very sure that these are significant...

26 May 2012 3,193 5 View

Change Detection

Is there a Change Dectetion tool in ArcGIS?

14 May 2012 4,732 2 View

Combine tool in arcgis

I am using the "COMBINE" function with 2 raster sets in ArcGIS. But the resultant attribute table has some missing rows with respect to the Field ID. Am I missing any specific "Environments"...

16 April 2012 632 12 View

Editorial delays in review process

I submitted a manuscript in the first week of September, 2019 and as of today, my manuscript is still with the editor. I wrote to a polite email to the editor asking for its progress....

01 January 1970 5,027 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View