How can I express a protein which contains four rare codons viz. Arg, Leu, Ile and Pro in its sequence?
I am expressing Leishmania protein in bacterial system. This sequence has four rare codons of proline, isoleucine, leucine and arginine. I have tried BL-21 DE3, BL-21 Gold, BL-21 RIL, BL-21 RP,...
14 February 2015
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