Ideas about STEM as a holistic discipline and STEM as separate disciplines in asian context
I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...
18 February 2021 1,450 3 View
The Folin-Ciocalteu method is an electron transfer based assay, and gives reducing capacity, which is usually expressed as phenolic content. Can anyone share any reference paper where total...
13 February 2021 3,782 3 View
First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...
03 February 2021 9,085 4 View
Even after few repetitions..I am getting similar results. Is is due to organic components?
20 January 2021 9,279 6 View
Is there any manual guide to perform multicollinearity test between environmental (19 Bioclimatic) variables for MaxEnt modelling? Using all variables without removing the correlated one affects...
14 January 2021 3,807 7 View
05 January 2021 9,040 4 View
I am working on a career interest test which is based on paired comparison data. There are 12 dimensions in 6 groups. Each dimension from each group is compared with all dimensions of all other...
22 December 2020 8,186 5 View
Hello, I have been doing some transfection experiments for certain gene studies and wants to further do gene expression studies using qRT-PCR. Currently, I am using 7*10,000 cells per well for...
08 December 2020 6,981 5 View
Hello, I've to get a transient response of an IM. For that, I have to simulate a dynamic Model, but the catch is, 'I have to do it all in codes' (preferably with backward Euler's method). The...
21 November 2020 10,019 3 View
Hi i was trying to deposit the polystyrene nano fibers on aluminum substrate... Can anyone help me how to enhance the nano fiber's adhesion to the substrate? Like annealing pr vacuum drying ? Any...
16 November 2020 1,735 7 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
18 February 2021 4,214 3 View
Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...
16 February 2021 8,389 3 View
I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...
15 February 2021 8,982 1 View
I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...
13 February 2021 9,838 1 View
Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...
10 February 2021 2,895 4 View
since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot
03 February 2021 5,362 2 View
Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...
28 January 2021 8,765 4 View