Ideas about STEM as a holistic discipline and STEM as separate disciplines in asian context
I have a .text file for various FASTA sequence , and i want to convert these sequences into a numeric file which will be in .csv format. OR I want to extract physiochemical properties(features)...
25 July 2024 3,650 2 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
As the biaxial loading may implement additional hardening of the material during fatigue with an increase in the biaxiality ratio. What would be the effect of planer biaxiality on dynamic strain...
31 May 2024 9,104 0 View
Ethics
15 April 2024 6,778 1 View
The gromacs tool do_dssp (freeware) uses the dssp program for a graphical output. In it's -cs output (scount.xvg) it gives you the secondary structure in the following form: @ s0 legend...
28 March 2024 8,449 2 View
Hello! I want to know how to test the electrochemical stability of the electrolyte (2M ZnSO4 solution) I have measured electrochemical stability window of a electrolyte by 3 electrode...
26 March 2024 3,306 1 View
Dear all, Will you please let me know how to calculate the carbon, nitrogen, and phosphorus (C: N:P) ratio in wastewater? Is it advisable to measure total COD, nitrogen, and phosphate? or I...
05 February 2024 4,105 3 View
When OCP stabilizes in a given electrolyte there is formation of passivation layer on sample surface or it shows that at this potential corrosion occurs. Also my tafel Ecorr values are not comming...
04 February 2024 8,735 2 View
Stage Temp Time Temp/time 1 95°C 5min - 2 95°C-85°C -...
31 January 2024 1,925 0 View
Please explain the role of green Knowledge management in brief and its influences.
24 January 2024 6,248 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...
05 July 2024 4,197 1 View
Hi everyone, I've been working on differentiating human iPSCs to derive a pure and uniform culture of neural progenitor cells (NPCs). However, I'm encountering a significant issue during the...
01 July 2024 1,012 1 View
studi che esplorano l'interazione tra interculturalità, competenze digitali e educazione STEM/STEAM. Cerco esempi di progetti educativi o casi di studio che dimostrano come questi elementi possano...
24 June 2024 1,552 0 View
Can performing flow cytometry evaluation on cells from the digestion of tumor samples frozen in liquid nitrogen or at -80ºC negatively influence the evaluation of cell cycle and tumor stem...
17 June 2024 7,129 0 View
This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....
12 June 2024 7,269 5 View
I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...
06 June 2024 941 1 View
A stem cell can divide to produce 2 identical copies of itself, which is called self-renewal. Then what is proliferation in terms of stem cells. Or is this term 'proliferation' applicable to stem...
04 June 2024 6,245 4 View
Dear Community Members, I'm reaching out to you, especially to those specializing in the medical field, for information regarding clinical trials on patients who have suffered from the rupture of...
04 June 2024 1,465 0 View