Can you help me with design of PCR amplification of long GC-rich regions?

Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...

24 August 2020 4,035 3 View

Do you know about any questionnaires for children measuring their attitudes to science and scientists?

I am doing a qualitative study on children's attitudes to science and scientists before and after our science education intervention, we deliver to primary schools. I'd like to include a...

27 January 2020 8,001 3 View

Can anyone recommend a corpus of research articles written by English native speakers?

I would like to use it as a reference corpus. I know there is a SERAC corpus, but it does not seem to be available for public.

01 April 2019 5,612 6 View

Illumina NextSeq multiplexing 384 samples per run? Barcodes?

Hello, I would like to ask you if you have any experience with multiplexing of 384 samples per run on Illumina NextSeq. We are using SNP assay designed by Roche = SeqCap EZ Choice library with...

05 December 2018 4,041 3 View

Can I use Tris/HCl instead to Bis-Tris in the gradient elfo with the same working pH and MES buffer?

I try a new method for chloroplasts analysis and its my first experiment with gradient electrophoresis for membrane proteins. In the protocol is Bis-Tris used, in lab we have Tris-HCl, the working...

04 December 2017 4,831 3 View

How to report results for generalised linear mixed model with binomial distribution?

Hello, I am currently working on the data analysis for my MSc. project comparing probability of occurrence of a species between two different habitats using presence - absence data. Due to the...

29 October 2017 8,273 5 View

Mapping ChiP-seq data on HiC data?

Hello, I am a bit new to the ChiP-seq data analysis and I need to map them onto HiC data, is there any tool to do that or visualize that somehow? Either online or using R-bioconductor? Any...

22 October 2017 8,782 1 View

Any database/online dictionary showing clearly morphemes and syllables (division)?

Hello everyone. I am urgently looking for a database or dictionary that shows already fragmented words - fragmented into morphemes and syllables as well (not pronounciation). I need it for a...

16 September 2017 8,583 1 View

Characterization for human melanoma BRO cell line?

30 April 2017 8,515 3 View

How to remove polysaccharides from protein samples?

Good afternoon, I try to extract cell wall proteins from green macroalgae, I have tried different protocols and one of them included step of EGTA extraction, however this fraction is jelly like,...

08 February 2017 1,271 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

CT image preprocessing in python?

I have a 3D CT image of a car engine in raw form with no header information. When loaded into numpy array as 16 bit unsigned integer, I noticed that the values range between 0 to 52000. Is this...

15 February 2021 4,736 3 View

How to detect uv vis spectrum of nanoparticle under 300nm?

I try to make the indium nanoparticle and check the uv vis spectrum. I heard that In nanoparticle has surface plasmon resonance peak at 200~300nm. But most of solvent has absorption peak under...

14 February 2021 6,950 15 View