Hi there,
For my IP experiments i'd like to purchase Dynabeads™ Protein A and all that i need for immunoprecipitation. Ive seen they sell kits and you can even buy it individually. What is weird to me, is the fact that they dont sell the "Antibody (Ab) Washing and Binding buffer", and "Washing Buffer" individually. (https://www.thermofisher.com/order/catalog/product/10018D?SID=srch-srp-10018D#/10018D?SID=srch-srp-10018D)
So my question is:
What do you use for washing antybody binding and the washing steps?
Is PBS (+/-BSA) a good alternative for the binding step and PBS+0.01%Tween for washing?
Thank you in advance!
Hi Peter,
If you look into the stand-alone Dynabeads Protein A (#10001D) manual on the Thermo Fisher Scientific web page, all the required buffers are mentioned:
https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FDynabeadsProteinA_man.pdf&title=RHluYWJlYWRzIFByb3RlaW4gQQ==
There is one protocol for manual handling and one for use with the KingFisher instruments for fast (40 min) automated IP. Please reach out to me for further technical questions, [email protected]
I think this happens due to similarity. The products are similar. Or not useful. It depends on the protocol or the product. What do they say?
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