Good Mouse model? - FAQS.TIPS

More Sahar Tahvili's questions See All

How pure the sucrose for cryopreservation should be?

I'm using 10 and 25% sucrose buffer for cryopreservation after fixating the samples in PFA. I wonder how pure the sucrose should be? Does it need to be 99% pure or so?

09 October 2016 7,396 6 View

What are good mouse NK and NKT cell markers?

I'd like to look at the effect of a drug on NK and NKT cell populations. I read that one of the best markers is CD1d. But, would it be possible to get a clean gating by using CD19 to get read to...

02 March 2016 2,416 2 View

Tumor homogenisation method for cytokine measurement?

Does anyone have a protocol for tumor homogenization that is good for cytokine measurement in tumor extract?

31 December 2015 7,535 4 View

Intracellular staining for Flowcytometry?

A basic question; I know should fixate and permeabilize cells before staining for intracellular markers, I wonder if there would be still possibility for staining the same markers which are...

11 December 2015 2,662 5 View

Can anyone suggest literature to help with immunofluorescence images interpretation?

I am new to using immunofluorescence techniques and confocal microscopy. Can anyone kindly introduce a good reference to me which I can read and understand how to analyze immunofluorescence...

03 April 2014 9,642 4 View

Why does gelatin not move during electrophoresis in gelatin zymography?

I've started doing zymography. I know that during gel casting the gelatin is somehow fixed in the gel, but, why it does not move during electrophoresis, since gelatin is also a protein?

02 March 2014 6,371 5 View

Mouse lung fibroblasts color?

I've separated fibroblasts from mouse lung tissue, and put them in culture. After a month of being in culture, I noticed that they turned black, just like B16 melanoma cells. Do you have any idea...

31 December 2013 5,149 3 View

Does it ever happen to you that the cells appear to resist migrating toward specific chemokine instead of attracting towards it?

I'm doing transmigration assays in which I coat the transwells with HUVEC cells, add different chemokines to the lower chamber and the cells from mouse peritoneum (PEC) on top of HUVEC, and let...

05 June 2013 7,485 9 View

How long can I keep the prepared master mix before running the real time pcr reaction?

Does anyone know if its possible to prepare the master mix and add all in a real time pcr plate, put the plate in freezer or refrigerator and then run the reaction the day after? Does it affect...

04 May 2013 8,779 12 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View