How pure the sucrose for cryopreservation should be?

09 October 2016 7,345 6 View

What are good mouse NK and NKT cell markers?

I'd like to look at the effect of a drug on NK and NKT cell populations. I read that one of the best markers is CD1d. But, would it be possible to get a clean gating by using CD19 to get read to...

02 March 2016 2,369 2 View

Tumor homogenisation method for cytokine measurement?

Does anyone have a protocol for tumor homogenization that is good for cytokine measurement in tumor extract?

31 December 2015 7,484 4 View

Intracellular staining for Flowcytometry?

A basic question; I know should fixate and permeabilize cells before staining for intracellular markers, I wonder if there would be still possibility for staining the same markers which are...

11 December 2015 2,619 5 View

Can anyone suggest literature to help with immunofluorescence images interpretation?

I am new to using immunofluorescence techniques and confocal microscopy. Can anyone kindly introduce a good reference to me which I can read and understand how to analyze immunofluorescence...

03 April 2014 9,596 4 View

Why does gelatin not move during electrophoresis in gelatin zymography?

I've started doing zymography. I know that during gel casting the gelatin is somehow fixed in the gel, but, why it does not move during electrophoresis, since gelatin is also a protein?

02 March 2014 6,325 5 View

Mouse lung fibroblasts color?

I've separated fibroblasts from mouse lung tissue, and put them in culture. After a month of being in culture, I noticed that they turned black, just like B16 melanoma cells. Do you have any idea...

31 December 2013 5,099 3 View

Does it ever happen to you that the cells appear to resist migrating toward specific chemokine instead of attracting towards it?

I'm doing transmigration assays in which I coat the transwells with HUVEC cells, add different chemokines to the lower chamber and the cells from mouse peritoneum (PEC) on top of HUVEC, and let...

05 June 2013 7,440 9 View

How long can I keep the prepared master mix before running the real time pcr reaction?

Does anyone know if its possible to prepare the master mix and add all in a real time pcr plate, put the plate in freezer or refrigerator and then run the reaction the day after? Does it affect...

04 May 2013 8,723 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View