How pure the sucrose for cryopreservation should be?

09 October 2016 7,345 6 View

What are good mouse NK and NKT cell markers?

I'd like to look at the effect of a drug on NK and NKT cell populations. I read that one of the best markers is CD1d. But, would it be possible to get a clean gating by using CD19 to get read to...

02 March 2016 2,369 2 View

Good Mouse model?

Does anyone know a mouse model in which NKT cells play a major or at least important role?

01 February 2016 1,400 2 View

Intracellular staining for Flowcytometry?

A basic question; I know should fixate and permeabilize cells before staining for intracellular markers, I wonder if there would be still possibility for staining the same markers which are...

11 December 2015 2,619 5 View

Can anyone suggest literature to help with immunofluorescence images interpretation?

I am new to using immunofluorescence techniques and confocal microscopy. Can anyone kindly introduce a good reference to me which I can read and understand how to analyze immunofluorescence...

03 April 2014 9,596 4 View

Why does gelatin not move during electrophoresis in gelatin zymography?

I've started doing zymography. I know that during gel casting the gelatin is somehow fixed in the gel, but, why it does not move during electrophoresis, since gelatin is also a protein?

02 March 2014 6,325 5 View

Mouse lung fibroblasts color?

I've separated fibroblasts from mouse lung tissue, and put them in culture. After a month of being in culture, I noticed that they turned black, just like B16 melanoma cells. Do you have any idea...

31 December 2013 5,099 3 View

Does it ever happen to you that the cells appear to resist migrating toward specific chemokine instead of attracting towards it?

I'm doing transmigration assays in which I coat the transwells with HUVEC cells, add different chemokines to the lower chamber and the cells from mouse peritoneum (PEC) on top of HUVEC, and let...

05 June 2013 7,440 9 View

How long can I keep the prepared master mix before running the real time pcr reaction?

Does anyone know if its possible to prepare the master mix and add all in a real time pcr plate, put the plate in freezer or refrigerator and then run the reaction the day after? Does it affect...

04 May 2013 8,723 12 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View