Sahar Tahvili

10 Questions 17 Answers 0 Followers

Questions related from Sahar Tahvili

How pure the sucrose for cryopreservation should be?

I'm using 10 and 25% sucrose buffer for cryopreservation after fixating the samples in PFA. I wonder how pure the sucrose should be? Does it need to be 99% pure or so?

10 October 2016 7,380 6 View

What are good mouse NK and NKT cell markers?

I'd like to look at the effect of a drug on NK and NKT cell populations. I read that one of the best markers is CD1d. But, would it be possible to get a clean gating by using CD19 to get read to...

03 March 2016 2,405 2 View

Good Mouse model?

Does anyone know a mouse model in which NKT cells play a major or at least important role?

02 February 2016 1,435 2 View

Tumor homogenisation method for cytokine measurement?

Does anyone have a protocol for tumor homogenization that is good for cytokine measurement in tumor extract?

01 January 2016 7,524 4 View

Intracellular staining for Flowcytometry?

A basic question; I know should fixate and permeabilize cells before staining for intracellular markers, I wonder if there would be still possibility for staining the same markers which are...

12 December 2015 2,652 5 View

Can anyone suggest literature to help with immunofluorescence images interpretation?

I am new to using immunofluorescence techniques and confocal microscopy. Can anyone kindly introduce a good reference to me which I can read and understand how to analyze immunofluorescence...

04 April 2014 9,627 4 View

Why does gelatin not move during electrophoresis in gelatin zymography?

I've started doing zymography. I know that during gel casting the gelatin is somehow fixed in the gel, but, why it does not move during electrophoresis, since gelatin is also a protein?

03 March 2014 6,360 5 View

Mouse lung fibroblasts color?

I've separated fibroblasts from mouse lung tissue, and put them in culture. After a month of being in culture, I noticed that they turned black, just like B16 melanoma cells. Do you have any idea...

01 January 2014 5,135 3 View

Does it ever happen to you that the cells appear to resist migrating toward specific chemokine instead of attracting towards it?

I'm doing transmigration assays in which I coat the transwells with HUVEC cells, add different chemokines to the lower chamber and the cells from mouse peritoneum (PEC) on top of HUVEC, and let...

06 June 2013 7,473 9 View

How long can I keep the prepared master mix before running the real time pcr reaction?

Does anyone know if its possible to prepare the master mix and add all in a real time pcr plate, put the plate in freezer or refrigerator and then run the reaction the day after? Does it affect...

05 May 2013 8,761 12 View