Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

31 December 2019 7,308 6 View

ELISA of a very low concentrated protein, in human blood samples?

Hey Everyone, For the people with experience in biochemistry/ELISA. what do you do in order to prepare human blood to see measure a protein through ELISA that is not highly concentrated in...

05 June 2018 5,294 4 View

How to improve an indirect sandwich ELISA standard curve?

Hey everyone, For those that perform "homemade" ELISA, I have been titrating the different components of a sandwich ELISA. The most "acceptable" curve that I have obtained reaches an absorbance...

05 June 2018 1,543 12 View

chessboard or checkerboard experiments for indirect sandwich ELISA?

Hello, has someone done chessboard or checkerboard experiments for an indirect sandwich ELISA? Is it possible to titrate every antibody as it were used for an indirect ELISA??, I mean adding the...

02 March 2018 5,283 6 View

Iba1 antibody wako replacement??

08 September 2017 622 17 View

How improve the sensitivity of an ELISA?

Hy anyone, I am currently designing a home made sandwich ELISA for a PSD protein and I am facing the problem that in spite of having a nice standard curve with a recombinat-GFP protein, I achieve...

07 August 2017 9,846 11 View

Where to publish a scientific/technical paper in neuroscience?

Hi everyone, Could you please suggest me a Journal for publishing a paper that describe the establishment of a sandwich ELISA (TECHNICAL) but at the same time use that tool to measure the...

01 January 1970 2,294 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View