Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

31 December 2019 7,308 6 View

Good Biotinylated antibodies for indirect ELISA??

Hello everyone, Could someone please recommend me a secondary biotinylated antibody that does not bind to the plate inespecifically giving high background noise reaction? I appreciate your help...

08 September 2018 5,289 0 View

ELISA of a very low concentrated protein, in human blood samples?

Hey Everyone, For the people with experience in biochemistry/ELISA. what do you do in order to prepare human blood to see measure a protein through ELISA that is not highly concentrated in...

05 June 2018 5,294 4 View

How to improve an indirect sandwich ELISA standard curve?

Hey everyone, For those that perform "homemade" ELISA, I have been titrating the different components of a sandwich ELISA. The most "acceptable" curve that I have obtained reaches an absorbance...

05 June 2018 1,543 12 View

chessboard or checkerboard experiments for indirect sandwich ELISA?

Hello, has someone done chessboard or checkerboard experiments for an indirect sandwich ELISA? Is it possible to titrate every antibody as it were used for an indirect ELISA??, I mean adding the...

02 March 2018 5,283 6 View

Iba1 antibody wako replacement??

08 September 2017 622 17 View

How improve the sensitivity of an ELISA?

Hy anyone, I am currently designing a home made sandwich ELISA for a PSD protein and I am facing the problem that in spite of having a nice standard curve with a recombinat-GFP protein, I achieve...

07 August 2017 9,846 11 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View