Could you let me know if I can use this product
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate #1706515
http://www.bio-rad.com/en-us/sku/1706515-goat-anti-rabbit-igg-h-l-hrp-conjugate?ID=1706515
to detect the primary antibody? In a website, it says , Secondary antibodies are specific for the isotype (class) and the species of the primary antibody (for example, a goat anti-rabbit secondary antibody is an antibody generated in goat for detection of a primary antibody generated in a rabbit)
In order to detect a plant protein, what secondary antibody should I use?
It's depends on your primary antibody. This secondary will detect primary antibodies produced in rabbit so if your primary is rabbit-anti-proteinX you should be fine.
I have another problem, My primary antibody is this product http://www.enzolifesciences.com/ADI-SPA-818/hsc70-plant-monoclonal-antibody-1d9/Here is the information about this product
HSC70 (plant) monoclonal antibody (1D9)
Host:Mouse Isotype:IgG1 Immunogen:Recombinant spinach Hsc70 (Hsp73). Species reactivity:Porcine, Plant Applications:IP, WB Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
I will buy this secondary antibody:
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP: blotting-grade horseradish peroxidase secondary antibody conjugate
http://www.bio-rad.com/en-us/sku/1706516-goat-anti-mouse-igg-h-l-hrp-conjugate?ID=1706516
Will my secondary antibody react with my primary antibody? I am talking about doing colorimetric and then scanning. Thank you very much. Thank you so much.
The secondary should react fine.
HRP conjugates are usually used for chemiluminescence detection, for colorimetric you may want to consider alkaline phosphatase conjugated secondary.
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