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Questions related from Nguyen Ta
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
02 March 2021 10,700 3 View
Hi, I am going to use the extraction procedure from an article, however, I don't understand the bolded step. Could you please explain how I precipitate the protein in cold acetone and what the 5:1...
20 November 2018 8,588 7 View
Hi, I extract protein from beans. My extraction solution contains SDS, glycerol, Na2S2O5, TrisHCL and protease inhibitor. After adding the solution, I centrifuge the samples for 10-15 minutes....
17 November 2018 7,974 3 View
Hi , I am studying capillary electrophoresis, I have a silly question: To detect the separated glycans. are we using fluoresence only or MS only, or both? I am consfused that if only MS is used...
01 November 2018 2,867 1 View
So I was analyzing HSP70 in plants by Western blot. However the bands I got was at around 150 kDa instead of 78 kDa indicated by the product information. I realized I didn't heat the...
29 October 2018 4,631 4 View
Hi, I recently bought 15-well gels instead of 10-well gels by mistake. I was wondering if there is any differences between them that I need to notice and if it is necessary to get it replaced. The...
20 October 2018 2,446 0 View
I'm going to extract proteins from bean plants. The recipe for the extraction buffer is 125mM Tris -HCl pH 8.8, 1% (w/v) SDS, 10% (v/v) glycerol, and 50 mM Na2S2O5. I add 200uL of the buffer to...
24 September 2018 8,435 1 View
Could you let me know if I can use this product Goat Anti-Rabbit IgG (H + L)-HRP Conjugate #1706515 http://www.bio-rad.com/en-us/sku/1706515-goat-anti-rabbit-igg-h-l-hrp-conjugate?ID=1706515 to...
14 September 2018 584 3 View
Can someone provide me some information about detection of bands in WB by fluorescence? I'm trying to resolve my issue that I don't have any other means of quantitation.
01 January 1970 8,169 17 View
I was performed a kinetic assay of a fusion phosphatase with DiFMUP. I did this in triplicate and obtained a graph of substrate concentration versus initial velocity. However, the graph doesn't...
01 January 1970 7,896 1 View
What are methods of measuring kcat of an enzyme in vivo? Can we measure the enzyme's fluorescence to determine its kcat? If so, how can we achieve that? Thanks.
01 January 1970 6,080 2 View
I'm working on a project that develops a fluorescent probe for a group of enzymes. The probe will be selective to an individual protein because part of the probe is the protein sequence. The...
01 January 1970 223 3 View
pCAP-Based Peptide Substrates: The New Tool in the Box of Tyrosine Phosphatase Assays (nih.gov) My group is making a substrate-containing probe that has a lower Km than the substrate alone for a...
01 January 1970 6,118 3 View
Hi, My results suggest that the level of HSP70 decreases at 30 oC, but then increases at a higher temperature, 35oC. Assuming I'm doing pipetting correcly, what would be the reason for this trend?...
01 January 1970 1,197 0 View
Hi, I would really appreciate if you could help me with my question. So, after I add the HRP (Horseradish Peroxidase) Substrate to the membrane, is there any step before I use the scanner? I had...
01 January 1970 2,814 1 View
