Which primer pair is better for amplifying endophytic fungal communities in roots of trees while avoiding host amplification?

Which primer pair is better for amplifying endophytic fungal communities (metabarcoding with Illumina sequencing) in roots of trees while avoiding host amplification? I would greatly appreciate a...

03 September 2020 728 3 View

No title

Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

What is the correct interpretation for this association between changes in two variables?

Hello everybody! We are carrying out a research where we associate baseline to post-intervention changes between motivation and physical activity variables. My question is the following: In an...

24 March 2020 910 3 View

UMBRELLA SAMPLING: How can I calculate dG from a PMF curve in automated fashion?

1. I am processing my umbrella sampling simulations using the Weighted Histogram Analysis Method in Gromacs (gmx wham). However, I would like to report the mean dG and a standard deviation. To do...

15 March 2020 1,455 3 View

How do I calculate free energy of binding for ligand-protein complex using Bennett Acceptance Ratio (BAR)?

Hi, I am familiar with performing MD simulations using Gromacs on protein-ligand complexes. However, BAR is a new technique to me and I need specific clarification of the decoupling sequence...

12 March 2020 3,619 1 View

What are the most obvious difficulties when we expose the scientific findings to the general population?

When we have the opportunity to express our scientific findings in front of our colleagues we have the opportunity to talk with similar people, but; What happens when we have to speak in front of...

13 January 2020 501 4 View

Overlap PCR resulting into smear. Any idea of why?

I want to fuse 2 genes (4kbp each gene). I amplified both of them by PCR adding an overlapping region of 27 bp and gel extracted them. First thing that I don't like is that I can't quantify the...

17 December 2019 6,622 9 View

Where can I get a key to Saturniidae (silkmoths) genera?

I would like to know if there are taxonomic keys, besides the ones in Lemaire's books, for any group within Saturniidae family. UPDATE: Thank you all for your answers, Lemaire's books are indeed...

22 May 2019 2,124 15 View

What are the most important functions to know in SPSS for Psychology?

I am on a short deadline and need to learn how to use spss. The program is very large and I am unsure where I should spend my time the most. Chi-square & T-tests Correlations &...

21 May 2019 9,279 2 View

What statistical software are being recommended lately to carry out the confirmatory analysis of a questionnaire?

There are different software to carry out confirmatory analyzes, such as SPSS AMOS, which sometimes are insufficient for the publication requirements of some journals. What would be the most...

11 March 2019 5,216 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View