Giant clam larvae - veliger preserved in 10% formalin. Would this specimen good to view under SEM after fix in fixative Glutaraldehyde?

More Tzuen Kiat Yap's questions See All

Any available online databases with gene variant classification ?

Does anyone know any online open source or free sites where I can search or check on gene variant classification eg. ACMG (Benign , pathogenic, etc) besides ClinVar? Thanks

04 August 2022 7,776 0 View

Is there any POLE mutation positive control available?

Does anyone know how I can perform validation of POLE mutation detection ? Is there any PCR / sequencing assays or positive controls that I can use/purchase ?

25 May 2022 2,998 0 View

How to obtain a smooth and homogeneous carbon slurry for making electrodes?

Hi all, I'm trying to prepare a carbon slurry for making electrodes in the EDLCs. However, I can't seem to get a smooth and homogeneous slurry. Generally, the slurry is prepared by dissolving PVdF...

20 November 2021 315 2 View

Advantages of long-read over short-read sequencing in bacterial WGS?

Hi all, I have prior experience in using the Illumina short-read sequencing for bacterial WGS. Given the increasing interest and development on the applications of long-read sequencing...

21 June 2021 6,051 4 View

What causes the disappearance of the semicircle in Nyquist plot and scattering of data points at high temperature?

I was measuring the bulk resistance of a gel polymer electrolyte using electrochemical impedance spectroscopy. As shown in the Nyquist plot obtained at room temperature, supposingly the width of...

01 May 2021 2,727 1 View

Has anyone performed dual DNA and RNA extraction from FFPE tissue using an automated extraction system?

My lab is currently using the manual column method for dual extraction of RNA and DNA from FFPE tissue (from the same sample) for NGS . This is due to the limited tissue available. The Fisher...

04 April 2021 4,246 3 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 1,058 4 View

Can we use SPSS to conduct Confirmatory Factor Analysis?

The Management of Aggression and Violence Attitude Scale (MAVAS) was used to identify staff perceptions about the causes and management of aggression and violence. Previous research has validated...

14 August 2020 2,306 7 View

How to determine the number of cells before suspending them in 1x binding buffer in Annexin V FITC assay?

I am performing Annexin V FITC assay for adherent cells. According to the protocol described, the treated cells were trypsinised and collected, followed by washing them twice with cold PBS. The...

18 July 2020 8,954 5 View

Red seaweed but morphology like Dictyota sp?

Anyone can help ID this red seaweed.... commercial important in aquaria trade. It look similar to Dictyota spps, but in red colour. Growing layer upon layer.

06 April 2020 7,273 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How to fix cells with glutaraldehyde for immunofluorescent staining?

Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...

17 June 2024 3,434 5 View

How to evaluate the toxicity of wastewater using zebrafish embryos and larvae?

I am looking for a methodology to evaluate the quality of wastewater using zebrafish larvae and embryos. Test No. 203: Fish, Acute Toxicity Test generally describes the procedure, but I have...

16 June 2024 6,473 0 View

Feeding zebrafish larvae properly, so how many grams or milligrams of Artemia should be given to 6 dpf larvae?

According to standard guidelines, experts suggest proper feeding ratios to grow the ZF healthly.

15 June 2024 4,354 1 View

How many grams or milligrams of Artemia should be given to 6 dpf larvae?

Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?

13 June 2024 389 0 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

How can i prepare self healing hydrogel for MTT assay?

Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its...

11 May 2024 6,310 1 View

A good microscope for identification and pictures of aquatic larvae?

I am looking to get a microscope for the identification of larvae in aquatic invasive species work. Would like to use it for the identification of larvae for aquatic invasive species work....

18 April 2024 4,401 2 View

Why papain is easily cross-linked by glutaraldehyde, while bovine serum albumin, keratinase is difficult to be cross-linked?

Why papain is easily cross-linked by glutaraldehyde, while bovine serum albumin, keratinase is difficult to be cross-linked

17 April 2024 7,782 0 View