Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its material. i want to know that i should fix it with glutaraldehyde or not?
Ultimately, the decision to fix your self-healing hydrogel with glutaraldehyde should be based on a careful evaluation of these factors and any specific requirements of your experiment or assay protocol. If in doubt, it's always a good idea to perform a pilot experiment or conduct some preliminary tests to assess the impact of glutaraldehyde fixation on your hydrogel and assay results. 1 Compatibility with Glutaraldehyde. 2 Crosslinking: 3 Assay Sensitivity 4 Protocol Requirements: Consultation
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