Helloo, maybe someone here can help me. i have acellline which gives me at baseline a clear FITC signal, i did not perform any transfection/modification to this line. can you explain this?
I placed a pulsating soft coral (Xenia umbellata) polyp under the microscope to look at the dinoflagellate symbionts in them. Then I noticed that there are also these darker pink blobs amongst the...
03 November 2021 2,118 1 View
Dear all, i have been trying to isolate microglia of adult murine brain . i used a percoll density gradient; unfortunately it worked only in the first time for me, very well. after that.. it...
11 August 2021 5,508 5 View
Mediator is negative, but moderator is positive, how? These were the results of the mediator analysis: X - M is significant positive M - Y is significant negative My indirect effect is negative,...
15 June 2021 1,067 4 View
I am hoping to estimate energy expenditure (kJ or calories) from actigraph reported movement behaviors (i.e. LPA, MVPA, SB) in minutes, for children age 8-12 years. The data was collected with the...
01 June 2021 2,545 0 View
Hello! I need to simulate and investigate possible chemical interactions between some polymers and dye molecules. Is there any free reliable software you would recommend? Thank you in advance....
29 January 2021 1,012 2 View
Please, i need urgent help. do you know where to buy Acrolein? Sigma used to sell this, but it is not available anymore. i checked elsewhere, but without success. please help! thank you silke
22 September 2020 3,291 8 View
Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...
29 July 2020 6,370 5 View
I am working on a coral reef monitoring project. The aspect I am interested in is assessing bleaching particularly in soft corals. Which kinds of approaches are sensible? Chlorophyll...
13 April 2020 4,078 0 View
I have encoded log2FC by size in STRING networks built with cytoscape (3.7.2). When exporting them, the log2FC key is not comparable across different networks as cytoscape exports either the...
09 April 2020 5,127 0 View
hello, i need some help. i am searching for good reference values for pediatric laboratories for the potassium levels. in the newest version of "Thomas" i found a good table, but they did not...
05 February 2020 3,583 6 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View