Is there any free reliable software to simulate chemical interactions between polymers and other molecules?

Hello! I need to simulate and investigate possible chemical interactions between some polymers and dye molecules. Is there any free reliable software you would recommend? Thank you in advance....

29 January 2021 541 2 View

Quantitative research - hypothesis formulation necessary?

Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...

29 July 2020 6,145 5 View

Potassium levels in pediatry?

hello, i need some help. i am searching for good reference values for pediatric laboratories for the potassium levels. in the newest version of "Thomas" i found a good table, but they did not...

05 February 2020 3,396 6 View

Hey! PLease help me out: do yoou know what happens if you add more Sodium azide in your primary antibody dilution? f.ex. 0,1%? any experience?

will the antibody binding be affected? (recommended is 0,02-0,05%) i would be happy to get some help

30 January 2020 7,624 1 View

How can you do statistics with normalized data?

Just briefly: I have WB quantifications from 4 different blots (4 biological repilcates) and I normalized my signal intensity values to the untreated ctrl sample. Therefore, for all 4 replicates...

14 July 2019 3,258 3 View

Internal normalization in Western Blot quantification and statistics. How to?

This might seem like a rather simple problem, but I am actually unable to find a helpful solution that specifically refers to this problem. I did several Western Blots of biological replicates...

10 July 2019 6,476 5 View

Is AmberliteXAD2 an suitable alternative for Bio-Beads?

We are currently looking for cheaper alternatives for removing detergent from proteins during reconstitution into membranes. Bio-Beads work nicely for us, but they are costly. We found now...

15 May 2018 559 2 View

Anyone knows someone interested in a PhD at the TUM in my Chronobiology/Immunology/Microbiota/Gut -group?

Thankful for any help!

24 April 2018 3,291 8 View

Can I selectively remove host cells from mouse blood while retaining intact bacteria?

To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...

22 October 2017 6,278 3 View

Preservation of bacterial transcriptome upon cooling?

Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...

12 September 2017 2,450 2 View

HPLC_MS or GC_MS analytical method for acrolein, without sample pretreatements?

Can anyone suggest a GC-MS or HPLC-MS analytical method to identify Acrolein in a solid sample (soluble in ethanol), without any pretreatement of the sample (no purge and trap, no SPME)?

24 March 2020 3,223 9 View

How can I treat my cells w/ aldehyde (acrolein) and let they in incubator withou harm the other cells that are in the incubator too?

When I treated my cells with acrolein (less then 1mM) (in a 12 well cell culture cluster) the other cells that were in a tissue culture flask 25 (growing normally without any treatment) were...

03 April 2019 7,557 4 View

The Diels-Alder reaction of 2,3-dimethyl-1,3-butadiene (DMB) and acrolein produces 3,4-dimethyl-~3-tetrahydro-benzaldehyde?

This overall second-order reaction was performed in methanol solvent with equimolar amounts of DMB and acrolein (C. R. Clontz, Jr., M.S. Thesis, Univ. of Virginia, 1997.) Use the data shown below...

01 February 2018 7,291 0 View

How long is sectioned brain tissue good for something?

Bird brains cryosectioned and kept in cryoprotectant in a -20C freezer for ~5 years. It was preserved with acrolein and some sections were used for IHC right away. What can I do with the rest of...

23 October 2016 9,173 6 View

Which GC column should i use for aqueous sample of glycerol?

I have a liquid sample containing water(around 70%) and rest is acrolein, glycerol, acetaldehyde. how can i detect them? I mean which GC column should be perfect for it?

28 June 2016 8,698 3 View

Any advice on cell test with evaporable aldehydes (formaldehyde, acetaldehyde and acrolein)?

09 October 2015 3,657 3 View

Hormesis and Transhormesis in Toxicology and Risk Assessment. Is a little bit of bad good for you?

Dear Colleagues, To prevent the toxicity of chemicals, ideally we should minimize our exposure. The best case scenario, of course, is to have no exposure at all, but, in the real world, this is...

01 February 2015 6,554 2 View

Does anyone study with acrolein?

I want to work with acrolein. But i don't know this product soluble in water or not

09 October 2014 7,900 1 View