ANOVA or Paired samples t-test?

Hi, I'm a masters student struggling with the design of my cognitive psychology project. We wish to run an experiment where we examine false memory recall using a list of schema related nouns in...

13 February 2021 6,457 3 View

What is the optimum Wolbachia population that can significantly affect dengue cases in densely populated provinces in the country?

I am working on a paper that looks into the dynamics of the spread of Wolbachia and its potential impact on dengue transmission, particularly in the Philippines.

29 October 2020 6,723 3 View

How to tackle autofluorescence using the technique FISH in mouse frozen tissue?

Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...

23 September 2020 3,849 1 View

How to customize a 'plt.var' plot from CCA package?

Hello, I'm trying to customize a CCA biplot made with the plt.var function from the CCA package. I need to reduce the size of the labels or be able to move those labels because they overlap on...

31 July 2020 2,938 3 View

What are the effects of Covid 19 on nursing students and faculties?

Due to crisis of Covid 19 students have to disconnect from their education system and for preparing nurses its must to relate the theory with skill labs and then hands on practice is required to...

17 May 2020 7,462 21 View

How does hypertonic tissue appear in sliding filament theory & how do the number of myosin heads in a hypertonic area affect muscle strength?

30 March 2020 2,169 5 View

Some participants completed their demographic details and some other questions but did not answer ANY of the scales. What should I do?

Should I include their demographic information in the demographics section Of my sample? Or should I delete them? Do I need to report on this?

01 October 2019 8,146 2 View

What are the factors that affect the compatibility between filler and solvent in fabrication of mixed matrix/composite membrane?

I've heard of compatibility between polymer and solvent used to dissolve it based on the solubility parameter. However, little have I found upon solvent and filler interaction and compatibility....

18 August 2019 5,376 1 View

Analytics of Egress from the location of a Fire?

Hi All, A student of mine is gathering data on what people do when encountering a fire in the home. This data will then be analysed. There is much research on what happens when the fire occurs in...

17 June 2019 3,030 2 View

Can a fish biologist help identify this species from Madagascar?

This animal was caught by a fisherman in northwestern Madagascar, about a month ago. It had a long, thin, cylindrical body, at least 0.9 m in length, and appeared not to have any scales. The head...

06 November 2018 3,459 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View