How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

Sample size in G mellonella studies?

Hi everybody, I am working with G mellonella, and I know that one of the advantages of it is the lack of ethics commit and the 3R principles. However, I am very insecure about the sample size I...

29 October 2020 2,079 1 View

What is considered a good binding affinity energy value between macromolecules?

Hi everyone, Does exist any threshold for binding affinity value between macromolecules? E.g. Any value lower than -5.0 Kcal/Mol..... How to determine that the binding affinity among different...

22 September 2020 4,329 3 View

How to perform phase transformation modeling on ABAQUS?

Hello everyone, I am modeling an AM process called SLM, on which a laser scan a powder bed in order to build a part. I am currently having problem with the phase-transformation phenomena (from...

06 April 2017 7,623 4 View

Is there any protocol for defined media for macrophage cultures?

I want to perform an infectivity assay to test a metal compound and I need to guarantee that the medium hasn't it in a free form (apart from proteins). There is any protocol using a defined media...

19 January 2017 2,973 3 View

Should I be happy if my data gives me a null hypothesis?

I mean, if it contradicts the current literature it might be even more relevant than a positive hypothesis, but my subject in particular is already controversial. People everywhere are getting...

02 October 2016 3,052 5 View

Hello, I am using GAP43 (B-5) mAb and found 2 bands with around 50 and 39Kda in SH-SY5Y cells. Which one should I measure as a GAP43?

Antibody from Santa Cruz

07 August 2016 2,256 2 View

How do I teach Inertial Reference Frame on the High School Level: ?

12 October 2015 3,551 8 View

How can I compare my infected human sample results from western blot versus ELISA?

06 July 2015 1,081 5 View

Is there any good literature for Cost-Effectiveness Analysis in Health?

Is there any good literature for Cost-Effectiveness Analysis in Health (specially oncology and Rad Onc)?Suggestion of books or articles

14 December 2014 7,645 10 View

What is the chemical composition of chlorellin?

The chlorellin is the chemical compound which is first extracted from Chlorella vulgaris by Pratt in 1944. Spoehr in 1949 wrote that it is a mixture between fatty-acids and hyrdocarbons and its...

25 January 2021 8,538 1 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View

Computation of p-value of time series using linear fitting of highly seasonal data. Should I remove the seasonal components prior to computation?

I have a time series of monthly chlorophyll-a observations that spans from 1997 to present day. I have computed the p-value of the time series without removing the seasonality, and it doesn't...

19 May 2020 3,259 5 View

How to identify pure green microalgae biofilm on SEM image?

I have SEM image of green microalgae Chlorella sp but I am not sure whether the green microalgae is pure or contaminated with other microbes.

18 February 2020 1,476 4 View

Why normalised fluorescence line height is so strange in most recent years?

I have been exploring data in the Agean sea from MODIS product normalized fluorescence line-height (nFLH). In many control areas, I have noticed a strange pattern on the fluorescence line-height...

01 February 2020 7,440 1 View

Help-me identify this genus of phytoplankton?

11 December 2019 7,761 4 View

Could someone tell me if this is phytoplacton, if so which?

11 December 2019 7,066 3 View

What could be a justification for using more than one biodiversity index?

For my project looking at the biodiversity of phytoplankton in the Arctic, I have calculated Hill numbers and Average Taxonomic Distinctness on my samples, and they both tell very different...

07 August 2019 3,029 3 View

How can i extract protein from microalgae (particularly spirulina and chlorella)?

Hello all, I need a facile method to extract protein from microalgae with a high yield. I tried HCl and NaOH solution along with sonication . But the yield was low. Would you have any suggestions...

06 July 2019 5,880 5 View