Genotyping mice with Neomycine?

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Etabs model stuck at analysis in pdelta,any solutions?

The model has no warnings, i tried searching for any unconnected frames and didn't find any. the model runs if i disabled pdelta.

02 October 2023 7,025 3 View

What is the Heat Dissipation of NMR ?

To start of I am designing an HVAC system where for a laboratory application utilizing Nuclear Magnetic Resonance operating at 300 MHz. I am well aware of the helium cooling within nitrogen shell...

29 March 2023 7,231 1 View

How to prepare a polysiloxane emulsion?

I would like to prepare a water-based emulsion with polysiloxane. What are suitable surfactants? What is the mixing procedure/conditions?

24 May 2022 2,549 3 View

Looking an open-source simulator for SDN (Mininet) and Blockchain (Ethereum) ?

Dear, I need your help, please. Could someone have an idea on an open-source simulator for SDN (Mininet) and Blockchain (Ethereum)? Thanks.

24 August 2021 3,734 3 View

Do you think there is a correlation between vitamin D insufficiency and early dental implant failure ?

Vitamin D acts as a hormone and is vital for the health of the brain, for the cardiovascular and respiratory tract, the skin, and the immune and endocrine systems. It is well known that vitamin D...

06 May 2021 2,143 3 View

What is the best way to transfect DNA into cells isolated from mouse mammary PyMT tumors?

I am planning on using electroporation by nucleofector kits, however I do not know which kit or protocol to use. Also, if you know of any other methods to transfect PyMT tumor cells please let me...

28 May 2018 2,558 3 View

What are the weak and strength points in Wood Cock Johnson Ach IV test?

Educ 6782 Assessment and Evaluation course

03 September 2017 2,079 1 View

Where can i find an Explanation for Queuing theory ?

1- Single-Server with Deterministic Arrivals and Services 2- Single-Server with Random Arrivals and Constant Service Rate 3- Multiple Servers

01 August 2016 5,919 4 View

How to conduct a crystal violet assay for quantifying biofilm?

Hello, I am new to this field of microbiology. I need someone's help in helping me develop the crystal violet assay in my lab. I am trying to develop a biofilm layer in a drinking water system...

26 June 2016 4,804 7 View

Droop Control - How can I regenerate the reference signals to be used in the inner control loop?

After applying the droop equations, I can find the load angle. I need three reference signals of the three phase voltage to use them in the inner control loop. Can i say that V reference = E(from...

30 April 2016 7,834 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View