In the original geNorm VBA applet, the raw Ct values were required to be converted in the relative Ct values (by using PCR efficiency and lowest Ct among samples). Does 'selectHKs' function in 'NormqPCR' package also require conversion of raw Ct values to relative values in the .txt files before analysis? OR Can I go ahead directly with raw Cq values?
Thanks,
If you add the raw Ct values you need to set log=TRUE.
The selectHK method calls stabMeasureM. This method calculates dependend on log:
if(log)
A
I am searching housekeeping genes using NormqPCR package. In the package, I wish to use geNorm function only. Can you teach me on how I should organize my raw Ct? Can you share with me the R script just to use geNorm function?
Dear Azmir,
You could see its vignette which completely describe Normqpcr package and GeNorm function.
according to its manual you could organize your data same as attached file.
best regards
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