The objective is to simply quantify the protein by Bradford method. Is it possible to just homogenize the tissue in water, centrifuge it and analyse the supernatant with Bradford's reagent?
I don't see why not, as long as you have no other plans for the sample. You may get a different result depending on the extraction solution you use. Most salts and buffers do not interfere with the Bradford assay, by the way. Detergents do interfere.
I don't see why not, as long as you have no other plans for the sample. You may get a different result depending on the extraction solution you use. Most salts and buffers do not interfere with the Bradford assay, by the way. Detergents do interfere.
As Adam wrote, you will get somewhat different results but you should be able to extract at least something (also depends on the tissue type).
Hi Karam, it is definitely possible, but from my experience with extracting proteins from fish tissue, corn and beans, using 0.5M NaCl in an extraction buffer (PBS or TBS) will increase the protein yield.
Don't forget to add proteinase inhibitors.
According to my experience it is possible, but the total yield will be increased if you use physiological buffer solution as recommend by many authors.
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