Genomic DNA or cDNA cloned in an adenoviral vector?

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Does anyone have any idea what Lysis Buffer from Beta galactosidase detection kit -ABCAM contain?

Hello! I have a question about a component from the Beta Galactosidase detection kit (Fluorometric)- AB176721 from ABCAM. From all the components of the kit, Lysis Buffer was used faster than the...

25 September 2022 201 1 View

How do you assess your students' individual progress in ESP during the COVID crisis-triggered online teaching process?

What type of testing methods have you devised in order to assess your students' progress in foreign languages in general or in ESP in particular? In my opinion, it's not only about their newly...

09 April 2020 932 1 View

What innovations are there in the prevention and control of decubitus ulcer?

Reaching out to my peers in hsr & healthcare innovation: I am looking for innovative (preferably tech-driven) tested solutions for the prevention and control of decubitus ulcer applicable in...

25 March 2019 4,515 3 View

How to take care of the athymic nude mice pups after birth?

We kept the pairs together until the pups are weaned. Some of the pups died and were eaten by the adult mice. What can we do to take care of the new born pups?

28 March 2018 4,367 7 View

Which is the best method for acid digestion when performing ICP-MS analysis on wood ash?

I want to determine the heavy metal contamination of wood samples by ICP-MS. I have calcined the sample following the EN 14775(2010) method (each wood sample was heated at a rate of 4.5 °C/min. i....

01 October 2017 2,553 4 View

Bioinfomartics - ABCC4 orthologs and homologs

Hi there, I am new to Bioinformatics and I am working on a project that requires me to identify orthologs of ABCC4. I have been using Uniport database, and have been identifying many redundant...

13 July 2017 8,460 2 View

Could anyone give me some information on hypobiosis and the metabolism of hypobiotic larvae?

I would be interested to find out how can a drug reach the arrested larvae of a nematode. Thanks

14 March 2016 690 2 View

How to avoid the tanglement of the preplaced sutures on the Modified Capsular Tension Ring suturing eyelet during the dialing process into the eye?

Cataract Surgery

06 March 2016 7,635 2 View

HIF-1 staining protocol?

I am trying to use HIF-1 to identify hypoxia in sections of 3D neuronal cultures. I have PFA fixed cells and sections and I cannot make any antibody work. I know for sure I have hypoxia as the...

17 August 2015 9,419 2 View

What is the typical range of delta 13C in natural organic matter from surface water and ground water sources?

I am looking at disinfection by-products formation and trying to make sense of some 13C abundance data I have collected. I would like to compare my data with data from other sources and have not...

28 May 2015 4,572 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

N. Max Schabla

cDNA derived from mRNA for your gene of interest is best. The genomic DNA of most genes contains introns which would make amplification for cloning difficult.

Tausif Alam

cDNA

Latif ur Rehman

If there is no intron then both cDNA and gDNA are OK, otherwise you have to use cDNA for gene expression

Anca Gafencu

Thank you for your answers!