05 December 2013 1 7K Report

I am looking for a web-based or computer based software which can be utilised to draw kind of a flow chart which graphically represents the zygosity and disease condition among the family members as seen in the attached pics.

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I have few sequence alignments (ClustalW) in .nex format and I simply need to construct a phylogenetic tree using MCMC. Please note I am not a bioinformatic guy nor familiar with all the...

28 June 2014 5,873 6 View

What is the best mutation analysis software to analyse sample sequences with a RefSeq?

I'm looking for a good software (free and/or commercial) to analyse my sample sequences with a refseq and see if there are any mutations, or the translated protein is affected. The software should...

23 June 2014 2,779 8 View

What is the best and most convenient way of storing buffy coat?

Is it even possible to restore and reuse buffy coat for PCRs and also for flow cytometry?

06 June 2014 3,269 3 View

Can this Qiagen Nuclease-Free water (Cat# 129115) be used for PCR?

I understand that the description of the product says it's suitable for any molecular biology application. The only doubt I have is that, most of other reagent companies only offer like 100ml for...

05 June 2014 4,001 4 View

How can I correctly calculate the melting and then annealing temperature of primers?

I've tried many web based apps available online to calculate the Tm and each one of them were producing completely distinct results which had caused me so much confusion. I've got 5 sets of...

29 April 2014 2,305 4 View

How to determine the optimum voltage and time for a better separation in gel electrophoresis?

I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, and do sequencing. My products of interest will be approximately around...

23 March 2014 6,074 20 View

Which is the simplest way to decide whether a mutation is pathogenesis or harmless?

I am planning to do a sequencing of a surface receptor on B cells and If I'll manage to find a novel mutation, I would like to know how to classify it as a pathogenic or harmless? (Simplest Way)

06 November 2013 9,442 24 View

Which is the best way to preserve whole blood for PCR?

I will have like 20 patients' blood samples in EDTA which I'd like to preserve for PCR later. I need to know for how long would it be possible to preserve that blood in EDTA and at which...

28 October 2013 5,850 15 View

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