20 Questions 23 Answers 0 Followers
Questions related from Harindra Sathkumara
I have calculated the relative expression ratio for my GOI between few different groups using REST 2009. I understand REST 2009, itself generates a Box-whisker plot and certain number of data...
09 November 2015 2,334 1 View
I was trying to do Bayesian analysis on some of my sequence data using BEAST 1.7.5 to see how closely related they are and their migration patterns. The substitution model used was GTR+I+G...
20 September 2015 8,625 1 View
Can you please explain to me how do we perform a mutational analysis and obtain a mutational rate using BEAST program. I have 10-15 sequences which I'd like compare. Thanks
01 January 2015 3,116 0 View
I have this tree constructed with BEAST and visualise with FigTree V1.4.1. I want to display two different values as the node label but I am not sure how exactly to do it. I'm a newbie to BEAST...
22 December 2014 597 0 View
I have found this mutation (silent - non-clinically significant) which I'd like to submit to dbSNP and get an ID. I have never submitted any variants to dbSNP. I guess I'm supposed to submit the...
20 October 2014 9,396 4 View
I have a couple of peptides (epitopes) with corresponding HLA alleles. Any possibility that I can visualise the structure of HLA alleles along with the bound epitope and to see how the epitope...
09 September 2014 5,947 7 View
Hi, I understand there are many protein structure prediction tools and what they produce are sometimes different from the actual x-ray crystallography results. Structure of one conserved domain of...
29 July 2014 8,091 14 View
Can any one send me a link to the Lasergene 11 mac installer? All I could find was the newer version 12 in their website. All I need is the installer, so I can use the license.
23 July 2014 4,095 1 View
I have few raw abi files and I'm trying to figure out if there are any heterozygous bases and also to do SNP/ variation detection using Geneious r7. I have attached the figures from Geneious where...
10 July 2014 5,806 1 View
I have few sequence alignments (ClustalW) in .nex format and I simply need to construct a phylogenetic tree using MCMC. Please note I am not a bioinformatic guy nor familiar with all the...
29 June 2014 6,222 6 View
I'm looking for a good software (free and/or commercial) to analyse my sample sequences with a refseq and see if there are any mutations, or the translated protein is affected. The software should...
24 June 2014 2,914 8 View
Is it even possible to restore and reuse buffy coat for PCRs and also for flow cytometry?
07 June 2014 3,369 3 View
I understand that the description of the product says it's suitable for any molecular biology application. The only doubt I have is that, most of other reagent companies only offer like 100ml for...
06 June 2014 4,183 4 View
I've tried many web based apps available online to calculate the Tm and each one of them were producing completely distinct results which had caused me so much confusion. I've got 5 sets of...
30 April 2014 2,397 4 View
I am planning to run some of the PCR products on a gel to identify the products of interest, perform a gel extraction, and do sequencing. My products of interest will be approximately around...
24 March 2014 6,190 20 View
I am planning to do a study where I'll be analysing few mutations and there contribution to a specific disease. All I need is to draw a pedigree chart where I might need to include and represent...
23 January 2014 5,489 1 View
I am looking for a web-based or computer based software which can be utilised to draw kind of a flow chart which graphically represents the zygosity and disease condition among the family members...
05 December 2013 7,236 1 View
I am planning to do a sequencing of a surface receptor on B cells and If I'll manage to find a novel mutation, I would like to know how to classify it as a pathogenic or harmless? (Simplest Way)
07 November 2013 9,529 24 View
I will have like 20 patients' blood samples in EDTA which I'd like to preserve for PCR later. I need to know for how long would it be possible to preserve that blood in EDTA and at which...
29 October 2013 5,937 15 View
I am intending to do a sequencing of a gene and to detect possible mutations in the coding region. I've seen many commercial software which are much more complex to use. What I need is a free,...
24 October 2013 3,994 5 View
