Generally we can amplify DNA by a dream Taq enzyme which can't be amplified by Pfu but I'm getting the opposite. What could be the reason?

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Daniela Ricci

In which buffer is your DNA sample? Is there EDTA? It could be that the conditions are fine for one enzyme and not for the other. Did you try a positive control for both the enzymes with the same buffer in which your DNA sample is?

Debaprasad Parai

Thanks Daniela. I have used 10mM Tris buffer to dissolve DNA sample. But I didn't try as you advised. I will check.