Debaprasad Parai

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Questions related from Debaprasad Parai

Which is the better procedure to revive a glycerol stock of bacterial culture stored at -80C?

Some people thought it is better to revive by inoculate them in broth. On the other hand many people suggest me to go for streaking method on agar plate instead of directly inoculate them in...

05 May 2014 2,807 63 View

Why do we use Tris solution of two different pH during preparation of SDS-PAGE?

Generally we use 1.5M Tris (pH=8.8) for preparation of resolving gel but 1.0M Tris (pH=6.8) for stacking gel. How does Tris having two different pH in a single polyacrylamide gel help to resolve...

09 September 2013 7,121 4 View

Generally we can amplify DNA by a dream Taq enzyme which can't be amplified by Pfu but I'm getting the opposite. What could be the reason?

How can I solve this?

08 August 2013 1,800 2 View