Anuradha Joshi

4 Questions 2 Answers 0 Followers

Questions related from Anuradha Joshi

Why is my plant DNA not amplifying in rbcL and ITS markers?

I isolated dried pressed herbarium plant samples using the modified CTAB method (Khanuja et. al., 1999) by adding PVP and beta-mercaptoethanol to reduce the number of polyphenols and...

13 February 2025 5,809 1 View

Is the CD1 in QIAGEN DNeasy PowerSoil Pro kit same as the CD1 in QIAGEN DNeasy Plant Pro Kit?

Is the CD1 buffer of QIAGEN a sodium thiocynate-based lysis buffer? Does it have any other detergents? Does anybody have the composition of CD1 buffer?

29 October 2024 3,921 0 View

Geneclean or Ethanol Precipitation, which is better for purifying low quality& low quantity DNA?

I have used the QIAGEN DNeasy kit to obtain fecal prey DNA from an herbivore and a few of the samples have higher polyphenol levels, which have given a yellowish tint to the final eluted DNA...

28 October 2024 7,480 2 View

How much percentage and thickness of agarose gel should I use?

I have three samples. One is a product obtained from CO1 primer with band of interest at around 350-380 bp, and two products obtained from ITS 1 & 2 primers with bands of interest at 340-375...

26 September 2024 7,148 3 View