Felipe Costa

5 Questions 4 Answers 0 Followers

Questions related from Felipe Costa

KIR genotyping? KIR Genes?

Help me define my KIR haplotype. I am starting to genotype KIR genes in a population. I'm unsure how to classify the haplotypes using standard PCR. Here’s an example of genes found in one person:...

05 November 2024 7,554 0 View

Does anyone understand the classification of KIR gene haplotypes based on the presence or absence of these genes?

31 October 2024 7,532 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

02 August 2024 5,084 4 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

25 July 2024 2,595 2 View

PBMC infection with virus in vitro?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. I would like to know...

01 January 1970 9,619 0 View