hi, I have 2 questions: does any one have upload a sequences of a bacteria genome with "N" for ambiguous characters to genbank ? if I need to submit without "N", it is ok if I just eliminate it using a script (like perl just to sustitute N with nothing) ?, it is valid for genbank?? ..... the genomes that I've submitted before I did not had that problem, but the ones that I'm trying to submit was impossible to eliminate it ("N") with the assembler software (cap3, vague, spades) that I used. second question: if I have contigs length less than 200 pb (the length tha genbank ask for), it acceptable to submit if I just eliminate those that are less than 200 pb (example: if the assembly results were 300 contigs, and 10 of them were less than 200pb, the submission to genbank will be 290 contigs, it is acceptable or ethical ) ???

Thanks so much