Does anyone can show me a structure of GAS5 LncRNA?
I need to examine the sequence of the bases of the three-dimensional structure of GAS5 LncRNA..
I would like to ask how to apply gravity load. When I apply gravity load in the different step, and in the same step with earthquake load, I got different floor response spectrum results. Which...
08 November 2023 3,593 0 View
I would like to ask how to apply different damping ratios in different direction. In modal dynamic analysis I can apply them with defining their own frequencies (1st mode for X direction, 3 mode...
08 November 2023 337 2 View
COVID-19 is a worldwide threat to humanity and spread fast. There is no effective vaccine yet, the combined antiviral therapies are performed and have a higher success rate. But we need additional...
05 July 2020 8,280 5 View
... There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. Here we report a detailed virological analysis of nine cases of COVID-19...
27 May 2020 9,812 4 View
Discussion killed by prejudice, please do not reply!!!
08 May 2018 4,505 20 View
Every member of the human population contains approximately 10131014 cells, 70% of which are cells of symbiotic bacteria predominantly localized in the gastrointestinal tract. Sometimes , I think...
18 April 2018 2,093 14 View
More than 2 years ago, Tomasetti and Vogelstein brought that question to prominence when they tried to sort out environmental versus inherited causes of cancer. They examined the extent to which...
21 January 2018 2,235 9 View
Sometimes , we use term of "zero time" in a formulation but are we sure it is really "0" ? maybe it is 0,000......1 and is there a "zero" time(can we stop the time?), or sometimes, we say v=0 are...
18 January 2018 8,890 74 View
According to the theory of inflation, the Universe grew faster than light and I think speed of inflation is "speed of time" at the same time.We are convicted of this inflation(time) with gravity.
12 January 2018 3,073 15 View
Accumulating evidence demonstrates that intestinal bacteria influence oncogenesis, tumor progression, and response to therapy. Thus, selectively manipulating the gut microbiota may represent a...
29 October 2017 7,434 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View