How can I recognize difference between anion exchange resin and cation exchange resin with FTIR analysis?
I want to know about the file which is attached.
I need to make a lysing solution to lyse the RBC and WBC for using in cell counter of sysmex k1000. When I use the blood control all parameter are normal but when I used the human blood the cell...
11 February 2024 8,536 0 View
I have a solution containig some salts and also Sodium Lauryl Sulfate (SLS). How can I identify the quantity of this surfactant?
13 July 2023 9,195 2 View
I used phase lock tubes for RNA extraction, but after adding chloroform and spinning down the gel came on top of aqueous layer! does anyone know why this might happen? and what should I do to...
25 April 2023 8,718 0 View
Dear fellows I am into micropropagation of calladium. we didn't observe any bacterial contamination during the initiation and first subculture stages. the second subculture was done last week and...
09 August 2022 6,391 3 View
I have a master with nanohole with diametre ~ 200 nm and a depth of 150 nm. i can not replicate the PDMS mold out from this master, every time PDMS is attached and fill the holes... Does anyone...
08 May 2022 1,143 0 View
-The atomic radius of lanthanum is approximately 0.35 nm, as opposed to 0.3 nm for water molecules. -Seawater sample also contain organic contaminants such as Mg, Na, etc. some of them with the...
02 January 2022 6,673 4 View
Hello everyone! I'm trying to make 2% MC, but after I autoclave the solution the water and the gel phase separate from each other! What can be done about this? Can I put it again on stirrer to...
21 July 2021 7,568 3 View
I am working on human fibroblast cells from synovial tissue and now they are in 4-5 passages. These cells have been confirmed with specific markers (99% purity). Recently, I have decided to...
17 November 2020 930 3 View
Diclofenac is separated by a nanofiltration membrane and the molecular size of diclofenac is needed to prove the mechanism of size exclusion. I did not find the molecular size of diclofenac...
29 October 2020 7,792 2 View
Hi dear fellows I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination. I tried ppm and antibiotics in media but both of them have...
20 September 2020 8,423 4 View
My thesis includes variables of inflation rate (monthly), interest rate (weekly), exchange rate (daily), and crude oil production (monthly), as well as imports and exports (annually). The period...
27 July 2024 7,384 5 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
Even adding fragrance is not working
05 July 2024 741 2 View