From which company I can get the HiB5 cell line?

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What are the average concentration of hormones especially estradiol in fetal bovine serum?

We have been using 10% FBS (Gibco) + MEM/F12 media for the study related with effect of estradiol in SH-SY5Y neuroblastoma cell proliferation. Now, the charcoal stripped FBS concept is confusing...

08 September 2018 6,673 0 View

Can MTT assay be taken as equivalent to cell proliferation assay (BrdU)?

Im currently working on SH-SY5Y cell line. The treated group showed increased BrdU +ve cells on Immunocytochemistry technique but % cell viability from MTT assay showed no significant effect. If...

08 September 2015 4,090 11 View

How should we analyze the two bands of phospho ERK1/2 in western blot ?

i got the two bands of 44kDa and 42kDa molecular weight of pERK1 and pERK2 respectively. Now i was wondering should we measure the two bands and compare it separately or take the ratio of...

07 August 2015 3,463 6 View

Are there any techniques, other than ELISA, for measuring secreted protein (21KDa) from the conditioned media?

Since ELISA kits are very expensive, was wondering if I could measure that with western blot technique? and If yes, how to measure the protein concentration? For the cell lysate I use protease...

03 April 2015 8,033 8 View

How to identify the cell debris with contamination in the cell culture?

After we thawed the cell line from liq N2 storage, we saw some floating things in the media after 24hrs, but after we change the media on that day we didn't see so much on the next day and didn't...

09 October 2013 6,798 25 View

BrdU or Nestin: which one is better to use as marker for proliferation assay?

Since both markers can be used to check the proliferation of neural stem cells, I was wondering what is the difference in using them? Is there a difference in the method using it or price? Can...

08 September 2013 3,879 8 View

Solvent for estradiol - can anyone help?

I saw some articles have used DMSO and some used ethanol as the solvent for estradiol and was wondering which should I chose and how does it make a difference to my results?

08 September 2013 3,853 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View