Rubina Shakya

8 Questions 13 Answers 0 Followers

Questions related from Rubina Shakya

What are the average concentration of hormones especially estradiol in fetal bovine serum?

We have been using 10% FBS (Gibco) + MEM/F12 media for the study related with effect of estradiol in SH-SY5Y neuroblastoma cell proliferation. Now, the charcoal stripped FBS concept is confusing...

09 September 2018 6,639 0 View

Can MTT assay be taken as equivalent to cell proliferation assay (BrdU)?

Im currently working on SH-SY5Y cell line. The treated group showed increased BrdU +ve cells on Immunocytochemistry technique but % cell viability from MTT assay showed no significant effect. If...

09 September 2015 4,059 11 View

How should we analyze the two bands of phospho ERK1/2 in western blot ?

i got the two bands of 44kDa and 42kDa molecular weight of pERK1 and pERK2 respectively. Now i was wondering should we measure the two bands and compare it separately or take the ratio of...

08 August 2015 3,425 6 View

Are there any techniques, other than ELISA, for measuring secreted protein (21KDa) from the conditioned media?

Since ELISA kits are very expensive, was wondering if I could measure that with western blot technique? and If yes, how to measure the protein concentration? For the cell lysate I use protease...

04 April 2015 7,981 8 View

How to identify the cell debris with contamination in the cell culture?

After we thawed the cell line from liq N2 storage, we saw some floating things in the media after 24hrs, but after we change the media on that day we didn't see so much on the next day and didn't...

10 October 2013 6,681 25 View

Solvent for estradiol - can anyone help?

I saw some articles have used DMSO and some used ethanol as the solvent for estradiol and was wondering which should I chose and how does it make a difference to my results?

09 September 2013 3,817 1 View

BrdU or Nestin: which one is better to use as marker for proliferation assay?

Since both markers can be used to check the proliferation of neural stem cells, I was wondering what is the difference in using them? Is there a difference in the method using it or price? Can...

09 September 2013 3,841 8 View

From which company I can get the HiB5 cell line?

I was trying to find the catalog number of this cell line and reliable source from where I can purchase it. Could any of you please help me provide this information regarding HiB5?

08 August 2013 2,694 2 View