Freezing hybridoma supernatant?

More Lorraine Mutsvunguma's questions See All

How can we improve the recruitment and retention of voluntary blood donors?

Enhancing the recruitment and retention of voluntary blood donors is vital for maintaining a safe and ample blood supply. Studies have indicated that the implementation of targeted recruitment...

09 April 2024 3,039 2 View

Is it possible to mix HRP anti-body with other anti-bodies to make them HRP conjugated?

If I need a secondary antibody: goat-anti-rabbit IgG HRP for a Western Blot, but only have goat-anti-rabbit IgG polyclonal, goat-anti-rabbit IgG Heavy Chain, and HA-Tag HRP could I mix any of the...

23 October 2023 6,047 0 View

What strategies can School Leadership Teams in the United Kingdom develop to enable fully inclusive school cultures in the UK? (SURVEY)?

If you are in a school leadership position in the United Kingdom, or an educational professional involved in teaching in UK schools or colleges , please contribute to my research survey/project...

17 December 2021 1,915 5 View

No amplification of SSR markers on Pecan nuts project?

Hi all, I am doing an SSR project on Pecan nuts and I am struggling to get amplification on my PCR. Here is a summary of what I have done so far 1. DNA extraction was a modified CTAB protocol...

19 April 2020 8,765 2 View

Ardales is reported as having a clay surface over rock being engraved, covered in calcite then eroded (also fluting) do you have more details of this?

I am focussing on clay in the Palaeolithic for my dissertation and reports on clay in Ardales are vague, including non-representative finger fluting and evidence for clay over a block being...

26 August 2019 9,583 0 View

Are there any research papers, models or tools which assist the clinician in assessing and differentiating ASD and psychosis?

I am currently looking into developing a tool which can be used clinically with young people 14yrs-25yrs who may present with both suspected ASD and psychotic symptomology. Before i begin i though...

10 September 2018 2,161 7 View

What would be a good material to to absorb high-frequency noise but not affect temperature?

Need to block high-frequency noise (ultrasound)from a datalogger that is recording temperature.

30 May 2018 4,264 1 View

Would you be interested to add South Africa in your research?

South Africa is a very diversed population with high level of inequality both in income and education. I have data available from 1850.

23 February 2018 5,075 8 View

By how much does the relative response factor of two isomers differ?

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Can the pre-conception maternal microbiome can exert an epigenetic influence on the fetal brain leading to autism spectrum disorder?

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21 February 2015 3,165 8 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

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How to Compress Information Neurally?

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How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

How to Freeze Embryos for Spatial Transcriptomics?

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10 July 2024 5,985 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

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What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?

02 July 2024 9,204 0 View

Ronald Van Vlierberghe

That is perfectly OK. We have done that a lot in the past.

Happy hollidays to you too :-)

With kind regards,

Ronald

Lorraine Mutsvunguma

Thank you Ronald!

Dominique Duval

usually there is no problem, but you may check before if the antibody you are harvesting resists to long term congelation (more than 1-3 months)